Azacitidine (DNA Methyltransferase Inhibitor)

Proposed Mechanism of Action

Azacitidine is a cytidine nucleoside analogue1 that is incorporated into newly synthesized DNA as well as RNA.1 Preclinical studies suggest that the antileukemic effects of azacitidine include direct cytotoxicity due to inhibition of protein synthesis and DNA damage and re-expression of aberrantly silenced tumor suppressor genes due to DNA hypomethylation.1-3

Azacitidine MOA Video

Azacitidine by Disease State

Azacitidine in Acute Myeloid Leukemia

  • Post Approval Research Acute Myeloid Leukemia AML (20%-30% blasts) (EU)
  • Post Approval Research Acute Myeloid Leukemia AML (>30% blasts) (EU)

Rationale for Clinical Development

Patients with acute myeloid leukemia (AML) have a poor prognosis, intensified by age-related factors and adverse disease characteristics.4-7 Intensive chemotherapy induction is standard treatment for AML but depends on patient fitness.6-8 The majority of patients who are ineligible or unfit for intensive chemotherapy are elderly,4,6-8 and their prognosis remains dismal.5 There is an unmet need for additional treatment options for patients with AML who are ineligible or unwilling to receive intensive chemotherapy.5 Preclinical studies in leukemia cell lines have demonstrated that treatment with azacitidine resulted in DNA demethylation, which supports its clinical investigation in AML.9

The safety and efficacy of the agents and/or uses under investigation have not been established. There is no guarantee that the agents will receive health authority approval or become commercially available in any country for the uses being investigated.

References

  1. Raj K, Mufti GJ. Ther Clin Risk Manag. 2006;2:377-388.
  2. Hollenbach PW, et al. PLoS One. 2010;5:e9001.
  3. Kaminskas E, et al. Oncologist. 2005;10:176-182.
  4. Pollyea DA, et al. Br J Haematol. 2011;152:524-542.
  5. Thein MS, et al. Cancer. 2013;119:2720-2727.
  6. Döhner H, et al. Blood. 2010;115:453-474.
  7. Milligan DW, et al. Br J Haematol. 2006;135:450-474.
  8. NCCN Clinical Practice Guidelines in Oncology. Acute Myeloid Leukemia. V1.2015.
  9. Hagemann S, et al. PLoS ONE. 2011;6:e17388.